Research Assistant

The Garcia Lab at Vanderbilt University Medical Center is seeking a motivated research assistant to join their efforts to study facial nerve disorders, facial paralysis, and nerve regeneration.  The role entails a variety of research tasks, ranging from assisting with mouse experiments, molecular biology, histology and microscopy work, as well as neuroscience and immunology assays. The Garcia Lab is focused on using cutting edge techniques to better understand facial paralysis as well as creating pre-clinical models of facial nerve injury for developing therapies that promote recovery from facial paralysis.  Our group focuses on neuro-immune interactions and hope to design immune-modulating therapies for promoting recovery after facial nerve injury.  Dr. Garcia is a surgeon-scientist trained in facial plastic and reconstructive surgery who specializes in disorders of the facial nerve.  As such, the research group emphasizes projects with immediate translational potential.

A successful candidate will be able to provide, under direct supervision, support for specified research projects; collect, assess, and communicate data/results. Assist in general laboratory operation and inventory.

Important technical skills:

Animal surgery, dissection, and tissue collection

Mouse colony maintenance

Mouse handling

Mouse behavior assessments 

Tissue processing and histology 

Immunofluorescence 

Image analysis

Molecular biology (qRT-PCR, western blot analysis, etc…)

Flow cytometry

Job Responsibilities:

1  .  Under moderate supervision, conduct and evaluate experiments and assays –

1a. Understand and follow experimental protocols (may include animal handling, western blotting, qRT-PCR and plate reader assays, mouse husbandry, etc.)

1b. Record, compile, interpret, and communicate data/results

1c. Follow University safety protocols (may include use of combustibles, radioactivity, toxic material, and/or carcinogens)

1d. Perform calculations involving molarity, % solutions, and serial dilutions

Oversee and conduct ongoing longitudinal mouse experiments

2. Prepare, operate and troubleshoot equipment in experimental procedures

2a. Read and follow all equipment manuals

2b. Maintain and repair equipment according to manufacturer and University guidelines

3. Ensure laboratory is prepared for experiments

3a. Order and receive supplies

3b. Maintain lab in a safe manner, following all regulatory requirements

4. Assist PI in modifying protocols/Demonstrate an understanding of acceptable procedures and techniques

4a. Recognize problems and suggest solutions

4b. Assist in modifications as directed

5. Provide appropriate written documentation of experiments

5a. Prepare preliminary research progress reports

5b. Summarize test results appropriately